View source for Tsien 1989 Methods Enzymol

{{Publication
|title=Tsien R, Pozzan T (1989) Measurement of cytosolic free Ca2+ with quin2.  Methods Enzymol 172:230-62.
|info=[https://pubmed.ncbi.nlm.nih.gov/2747529/ PMID: 2747529]
|authors=Tsien R, Pozzan T
|year=1989
|journal=Methods Enzymol
|abstract=The fluorescent Ca2+ indicator dye—quin2—is the basis of one of the most popular methods for measuring cytosolic free Ca2+ [(Ca2+]i], especially in suspensions of mammalian cells. Quin2 has several advantages over other methods for measuring cytosolic free Ca2+: (1) it can be loaded into the cytosol of millions of cells simultaneously without any micromanipulations or any disruption of the plasma membrane, (2) it is monitored with a conventional cuvette spectrofluorimeter, (3) its readings are usually easily calibrated at typical resting levels of [Ca2+]i (∼10−7M) and below (to 10−8M), whereas all other techniques do their best at detecting activated levels (∼10−6M) and are nearly at their detection limits at resting levels, and (4) it avoids many of the drawbacks particular to other [Ca2+]i measurement techniques. The chapter compares quin2 with newer fluorescent indicators—fura-2 and indo-1—as they are much brighter in fluorescence than quin2. Because fura-2 and indo-1 are sensitive enough to give large calibratable signals from single cells or regions of cells, they allow a direct measurement of compartmentalization and heterogeneity.
|keywords=calcium, quin2,
|editor=[[Cecatto C]]
}}
{{Labeling
|area=Instruments;methods
|topics=Calcium
|additional=2020-05, Ca-list
}}