Vincent 2015 Front Physiol
| Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Front Physiol 24:51. |
Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Front Physiol
Abstract: High-intensity short-duration interval training (HIT) stimulates functional and metabolic adaptation in skeletal muscle, but the influence of HIT on mitochondrial function remains poorly studied in humans. Mitochondrial metabolism as well as mitochondrial-associated protein expression were tested in untrained participants performing HIT over a 2-week period.
Eight males performed a single-leg cycling protocol (12 Γ 1 min intervals at 120% peak power output, 90 s recovery, 4 days/week). Muscle biopsies (vastus lateralis) were taken pre- and post-HIT. Mitochondrial respiration in permeabilized fibers, citrate synthase (CS) activity and protein expression of peroxisome proliferator-activated receptor gamma coactivator (PGC-1Ξ±) and respiratory complex components were measured.
HIT training improved peak power and time to fatigue. Increases in absolute oxidative phosphorylation (OXPHOS) capacities and CS activity were observed, but not in the ratio of CCO to the electron transport system (CCO/ETS), the respiratory control ratios (RCR-1 and RCR-2) or mitochondrial-associated protein expression. Specific increases in OXPHOS flux were not apparent after normalization to CS, indicating that gross changes mainly resulted from increased mitochondrial mass.
Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression. β’ Keywords: HIT, PGC-1Ξ±, mitochondria, oxidative phosphorylation, skeletal muscle
β’ O2k-Network Lab: NZ Auckland Hickey AJ
Labels: MiParea: Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
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