Weiss 2022 J Cell Mol Med

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Weiss SL, Zhang D, Farooqi S, Wallace DC (2022) Sodium butyrate reverses lipopolysaccharide-induced mitochondrial dysfunction in lymphoblasts. https://doi.org/10.1111/jcmm.17342

Β» J Cell Mol Med 26:3290-93. PMID: 35587004 Open Access

Weiss Scott L,  Zhang Donglan,  Farooqi Sumera,  Wallace Douglas C (2022) J Cell Mol Med

Abstract: Butyrate is a short-chain fatty acid that is produced by commensal microbes within the intestinal microbiome through fermentation of dietary fibre. Microbial-derived butyrate has been shown to promote immunologic and metabolic homeostasis, in part through its beneficial effects on mitochondrial function, and thus has been proposed as a possible anti-inflammatory therapy. We tested the hypothesis that butyrate could mitigate the decrease in mitochondrial respiration in immune cells under septic conditions as a preliminary step towards better understanding the potential for butyrate as a novel therapy in sepsis. Mitochondrial respiration and content (measured as citrate synthase activity) were compared within four Epstein-Barr virus-transformed lymphoblast (LB) cell lines exposed to either control media or lipopolysaccharide (LPS) 100 ng/ml. Both co-incubation of LBs with LPS + butyrate and treatment with butyrate after LPS stimulation reversed the decrease in mitochondrial respiration observed in LBs exposed to LPS without butyrate. Neither LPS nor butyrate led to significant changes in citrate synthase activity. The preliminary findings support further investigation of a potential mitochondrial-based mechanism through which butyrate may help to mitigate the immuno-inflammatory response in sepsis. β€’ Keywords: Human immunology, Immunometabolism, Metabolism, Mitochondria, Sepsis β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: US PA Philadelphia Wallace DC

Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Sepsis 

Tissue;cell: Other cell lines  Preparation: Intact cells 

Coupling state: ROUTINE, OXPHOS, ET  Pathway:HRR: Oxygraph-2k 


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