Wuest 2012 Am J Physiol Heart Circ Physiol

Wüst RC, Myers DS, Stones R, Benoist D, Robinson PA, Boyle JP, Peers C, White E, Rossiter HB. (2011) Regional skeletal muscle remodeling and mitochondrial dysfunction in right ventricular heart failure.. American Journal of Physiology, Heart and Circulatory Physiolgy Epub ahead of print.

Wüst RC, Myers DS, Stones R, Benoist D, Robinson PA, Boyle JP, Peers C, White E, Rossiter HB. (2011) American Journal of Physiology, Heart and Circulatory Physiolgy

Abstract: Exercise intolerance is a cardinal symptom of right ventricular heart failure (RV HF) and skeletal muscle adaptations play a role in this limitation. We determined regional remodeling of muscle structure and mitochondrial function in a rat model of RV HF induced by monocrotaline injection (MCT; 60 mg⋅kg(-1); n=11). Serial sections of the plantaris were stained for fiber type, succinate dehydrogenase (SDH) activity and capillaries. Mitochondrial function was assessed in permeabilized fibers using respirometry, and isolated complex activity by blue native gel electrophoresis (BN PAGE). All measurements were compared to saline-injected control animals (CON; n=12). Overall fiber cross-sectional area was smaller in MCT than CON: 1843±114 vs. 2322±120 μm(2) (P=0.009). Capillary-to-fiber ratio was lower in MCT in the oxidative plantaris region (1.65±0.09 vs. 1.93±0.07; P=0.03), but not in the glycolytic region. SDH activity (P=0.048) and maximal respiratory rate (P=0.012) were each ~15% lower in all fibers in MCT. ADP sensitivity was reduced in both skeletal muscle regions in MCT (P=0.032), but normalized by rotenone. A 20% lower complex I/IV activity in MCT was confirmed by BN PAGE. MCT-treatment was associated with lower mitochondrial volume density (lower SDH activity), quality (lower complex I activity), and fewer capillaries per fiber area in oxidative skeletal muscle. These features are consistent with structural and functional remodeling of the determinants of oxygen supply potential and utilization that may contribute to exercise intolerance and reduced quality of life in patients with RV HF. • Keywords: Bio-energetics, exercise, mitochondrial complex I, respirometry, monocrotaline

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Organism: Rat  Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.  Enzyme: Complex I  Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Oxygraph-2k