Difference between revisions of "Xia 2016 RSC Adv"
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{{Publication | {{Publication | ||
|title=Xia Z, Yi X, Zhuang Y (2016) Stable over-expression of the human malateāaspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2 . RSC Adv 6:61268-77 . | |title=Xia Z, Yi X, Zhuang Y (2016) Stable over-expression of the human malateāaspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2. RSC Adv 6:61268-77. | ||
|info=[https://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra06343h/unauth#!divAbstract Royal Society of Chemistry] | |info=[https://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra06343h/unauth#!divAbstract Royal Society of Chemistry] | ||
|authors=Xia Z, Yi X, Zhuang Y | |authors=Xia Z, Yi X, Zhuang Y | ||
|year=2016 | |year=2016 | ||
|journal=RSC Adv | |journal=RSC Adv | ||
|abstract=The considerable losses sustained by the pig industry due to porcine circovirus-2 (PCV2) could be avoided by using an attenuated vaccine. However, production of an attenuated PCV2 vaccine has not been satisfactory. Recently, we over-expressed the human malateāaspartate NADH shuttle member Aralar I in PK15 cells to enhance proliferation of PCV2. Compared with control PK15 cells, lactate accumulation in Aralar I over-expressing cells decreased by 44.8% (p < 0.01), whereas the mitochondrial NADH and pyruvate concentrations increased by 50% (p < 0.05) and 35.4% (p < 0.05), respectively. The ATP/ADP ratio and O<sub>2</sub> uptake rate also increased by 39% (p < 0.05) and 37.8% (p < 0.01), respectively. Furthermore, the glutamine consumption rate increased by 36.4% (p < 0.01). Finally, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05). These results revealed that over-expression of Aralar I can improve the energy metabolism of PK15 cells and also enhance the replication of the PCV2 virus in PK15 cells. This study presents a method to improve energy metabolism and provides a valuable thinking to enhance virus vaccine production. | |abstract=The considerable losses sustained by the pig industry due to porcine circovirus-2 (PCV2) could be avoided by using an attenuated vaccine. However, production of an attenuated PCV2 vaccine has not been satisfactory. Recently, we over-expressed the human malateāaspartate NADH shuttle member Aralar I in PK15 cells to enhance proliferation of PCV2. Compared with control PK15 cells, lactate accumulation in Aralar I over-expressing cells decreased by 44.8 % (p < 0.01), whereas the mitochondrial NADH and pyruvate concentrations increased by 50 % (p < 0.05) and 35.4 % (p < 0.05), respectively. The ATP/ADP ratio and O<sub>2</sub> uptake rate also increased by 39% (p < 0.05) and 37.8 % (p < 0.01), respectively. Furthermore, the glutamine consumption rate increased by 36.4 % (p < 0.01). Finally, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05). These results revealed that over-expression of Aralar I can improve the energy metabolism of PK15 cells and also enhance the replication of the PCV2 virus in PK15 cells. This study presents a method to improve energy metabolism and provides a valuable thinking to enhance virus vaccine production. | ||
|editor=[[Plangger M]], | |editor=[[Plangger M]], | ||
|mipnetlab=CN Shanghai Yi X | |mipnetlab=CN Shanghai Yi X | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Genetic knockout;overexpression, Pharmacology;toxicology | |area=Respiration, Genetic knockout;overexpression, mt-Medicine, Pharmacology;toxicology | ||
|diseases=Infectious | |diseases=Infectious | ||
|organism=Pig | |organism=Pig | ||
| Line 17: | Line 17: | ||
|topics=Ion;substrate transport | |topics=Ion;substrate transport | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=Labels, 2018-10, | |additional=Labels, 2018-10, Virus, | ||
}} | }} | ||
Revision as of 14:51, 23 February 2020
| Xia Z, Yi X, Zhuang Y (2016) Stable over-expression of the human malateāaspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2. RSC Adv 6:61268-77. |
Xia Z, Yi X, Zhuang Y (2016) RSC Adv
Abstract: The considerable losses sustained by the pig industry due to porcine circovirus-2 (PCV2) could be avoided by using an attenuated vaccine. However, production of an attenuated PCV2 vaccine has not been satisfactory. Recently, we over-expressed the human malateāaspartate NADH shuttle member Aralar I in PK15 cells to enhance proliferation of PCV2. Compared with control PK15 cells, lactate accumulation in Aralar I over-expressing cells decreased by 44.8 % (p < 0.01), whereas the mitochondrial NADH and pyruvate concentrations increased by 50 % (p < 0.05) and 35.4 % (p < 0.05), respectively. The ATP/ADP ratio and O2 uptake rate also increased by 39% (p < 0.05) and 37.8 % (p < 0.01), respectively. Furthermore, the glutamine consumption rate increased by 36.4 % (p < 0.01). Finally, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05). These results revealed that over-expression of Aralar I can improve the energy metabolism of PK15 cells and also enhance the replication of the PCV2 virus in PK15 cells. This study presents a method to improve energy metabolism and provides a valuable thinking to enhance virus vaccine production.
ā¢ Bioblast editor: Plangger M ā¢ O2k-Network Lab: CN Shanghai Yi X
Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine, Pharmacology;toxicology
Pathology: Infectious
Organism: Pig Tissue;cell: Kidney Preparation: Intact cells
Regulation: Ion;substrate transport
HRR: Oxygraph-2k
Labels, 2018-10, Virus
