Zhang 2018 Cardiovasc Diabetol: Difference between revisions
(Created page with "{{Publication |title=Zhang X, Zhang Z, Yang Y, Suo Y, Liu R, Qiu J, Zhao Y, Jiang N, Liu C, Tse G, Li G, Liu T (2018) Alogliptin prevents diastolic dysfunction and preserves l...") ย |
No edit summary |
||
Line 17: | Line 17: | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology | |area=Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology | ||
|diseases=Diabetes | |diseases=Cardiovascular, Diabetes | ||
|injuries=Oxidative stress;RONS | |injuries=Oxidative stress;RONS | ||
|organism=Rabbit | |organism=Rabbit |
Revision as of 13:22, 31 January 2019
Zhang X, Zhang Z, Yang Y, Suo Y, Liu R, Qiu J, Zhao Y, Jiang N, Liu C, Tse G, Li G, Liu T (2018) Alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function in diabetic rabbits. Cardiovasc Diabetol 17:160. |
Zhang X*, Zhang Z, Yang Y*, Suo Y, Liu R, Qiu J, Zhao Y, Jiang N, Liu C*, Tse G, Li G, Liu T (2018) Cardiovasc Diabetol
Abstract: There are increasing evidence that left ventricle diastolic dysfunction is the initial functional alteration in the diabetic myocardium. In this study, we hypothesized that alogliptin prevents diastolic dysfunction and preserves left ventricular mitochondrial function and structure in diabetic rabbits.
A total of 30 rabbits were randomized into control group (CON, nโ=โ10), alloxan-induced diabetic group (DM, nโ=โ10) and alogliptin-treated (12.5 mg/kd/day for 12 weeks) diabetic group (DM-A, nโ=โ10). Echocardiographic and hemodynamic studies were performed in vivo. Mitochondrial morphology, respiratory function, membrane potential and reactive oxygen species (ROS) generation rate of left ventricular tissue were assessed. The serum concentrations of glucagon-like peptide-1, insulin, inflammatory and oxidative stress markers were measured. Protein expression of TGF-ฮฒ1, NF-ฮบB p65 and mitochondrial biogenesis related proteins were determined by Western blotting.
DM rabbits exhibited left ventricular hypertrophy, left atrial dilation, increased E/e' ratio and normal left ventricular ejection fraction. Elevated left ventricular end diastolic pressure combined with decreased maximal decreasing rate of left intraventricular pressure (-โdp/dtmax) were observed. Alogliptin alleviated ventricular hypertrophy, interstitial fibrosis and diastolic dysfunction in diabetic rabbits. These changes were associated with decreased mitochondrial ROS production rate, prevented mitochondrial membrane depolarization and improved mitochondrial swelling. It also improved mitochondrial biogenesis by PGC-1ฮฑ/NRF1/Tfam signaling pathway.
The DPP-4 inhibitor alogliptin prevents cardiac diastolic dysfunction by inhibiting ventricular remodeling, explicable by improved mitochondrial function and increased mitochondrial biogenesis. โข Keywords: Diabetes mellitus, Diabetic cardiomyopathy, Dipeptidyl peptidase-4 inhibitors, Mitochondrial biogenesis, Mitochondrial function โข Bioblast editor: Plangger M
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology
Pathology: Cardiovascular, Diabetes
Stress:Oxidative stress;RONS
Organism: Rabbit
Tissue;cell: Heart
Preparation: Isolated mitochondria
Coupling state: OXPHOS
Pathway: N
HRR: Oxygraph-2k
Labels, 2019-01