Difference between revisions of "Zverova 2019 Neuropsychiatr Dis Treat"
Line 20: | Line 20: | ||
|diseases=Other | |diseases=Other | ||
|organism=Human | |organism=Human | ||
|tissues=Platelet | |tissues=Blood cells, Platelet | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|enzymes=Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase | |enzymes=Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase | ||
Line 26: | Line 26: | ||
|pathways=ROX | |pathways=ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2019-01, | |additional=2019-01, MitoEAGLE blood cells data, | ||
}} | }} |
Latest revision as of 23:45, 29 February 2020
Zvěřová M, Hroudová J, Fišar Z, Hansíková H, Kališová L, Kitzlerová E, Lambertová A, Raboch J (2019) Disturbances of mitochondrial parameters to distinguish patients with depressive episode of bipolar disorder and major depressive disorder. Neuropsychiatr Dis Treat 15:233-40. |
Zverova M, Hroudova J, Fisar Z, Hansikova H, Kalisova L, Kitzlerova E, Lambertova A, Raboch J (2019) Neuropsychiatr Dis Treat
Abstract: Mitochondrial dysfunctions are implicated in the pathophysiology of mood disorders. We measured and examined the following selected mitochondrial parameters: citrate synthase (CS) activity, electron transport system (ETS) complex (complexes I, II, and IV) activities, and mitochondrial respiration in blood platelets.
The analyses were performed for 24 patients suffering from a depressive episode of bipolar affective disorder (BD), compared to 68 patients with MDD and 104 healthy controls. BD and unipolar depression were clinically evaluated using well-established diagnostic scales and questionnaires.
The CS, complex II, and complex IV activities were decreased in the depressive episode of BD patients; complex I and complex I/CS ratio were significantly increased compared to healthy controls. We observed significantly decreased complex II and CS activities in patients suffering from MDD compared to controls. Decreased respiration after complex I inhibition and increased residual respiration were found in depressive BD patients compared to controls. Physiological respiration and capacity of the ETS were decreased, and respiration after complex I inhibition was increased in MDD patients, compared to controls. Increased complex I activity can be a compensatory mechanism for decreased CS and complex II and IV activities.
We can conclude that complex I and its abnormal activity contribute to the defects in cellular energy metabolism during a depressive episode of BD. The observed parameters could be used in a panel of biomarkers that could selectively distinguish BD depression from MDD and can be easily examined from blood elements. • Keywords: Affective disorder, Biomarker, Mitochondrial enzyme, Oxidative phosphorylation, Platelet • Bioblast editor: Plangger M • O2k-Network Lab: CZ Prague Fisar Z, CZ Prague Zeman J
Labels: MiParea: Respiration, Patients
Pathology: Other
Organism: Human Tissue;cell: Blood cells, Platelet Preparation: Intact cells Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase
Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k
2019-01, MitoEAGLE blood cells data