Difference between revisions of "Biological contamination"
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:::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux | :::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux in the [https://wiki.oroboros.at/index.php/Closed_chamber '''closed chamber'''] ([https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background Instrumental background flux ''J''°<sub>O2</sub>]), assessed in a [https://wiki.oroboros.at/index.php/Oxygen_sensor_test '''Q1: Oxygen sensor test'''] or [https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background'''QC2: Instrumental O<sub>2</sub> background test''']. | ||
:::: The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the O2k and using a strong acid. | :::: The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the O2k and using a strong acid. | ||
Revision as of 15:38, 11 August 2020
- high-resolution terminology - matching measurements at high-resolution
Biological contamination
Description
Biological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Biological contamination of the O2k-Chamber is detected as a high O2 flux in the closed chamber (Instrumental background flux J°O2), assessed in a Q1: Oxygen sensor test or QC2: Instrumental O2 background test.
- The problem can be solved by first cleaning the chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as MiR05. Make sure the 70% ethanol does not contain any additives; e.g., 70 % ethanol used in hospital settings may contain antiseptics (information provided by Garcia-Roves PM). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under Cleaning the glass chamber by removing the glass chamber from the O2k and using a strong acid.
