Difference between revisions of "Biological contamination"
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:::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux in the [https://wiki.oroboros.at/index.php/Closed_chamber '''closed chamber'''], measured in a [https://wiki.oroboros.at/index.php/Oxygen_sensor_test '''Q1: Oxygen sensor test'''] or | :::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux in the [https://wiki.oroboros.at/index.php/Closed_chamber '''closed chamber'''], measured in a [https://wiki.oroboros.at/index.php/Oxygen_sensor_test '''Q1: Oxygen sensor test'''] or '''QC2: Instrumental O<sub>2</sub> background test''' [https://wiki.oroboros.at/index.php/MiPNet14.06_Instrumental_O2_background MiPNet14.06]. | ||
:::: The problem can be solved by first cleaning the O2k-Chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics ([https://wiki.oroboros.at/index.php/Talk:MiPNet19.03_O2k-cleaning_and_ISS#Problem_solved EtOH containing antiseptics] provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [https://wiki.oroboros.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS O2k-Chamber cleaning with HCl] by removing the glass chamber from the O2k and using a strong acid. | :::: The problem can be solved by first cleaning the O2k-Chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics ([https://wiki.oroboros.at/index.php/Talk:MiPNet19.03_O2k-cleaning_and_ISS#Problem_solved EtOH containing antiseptics] provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [https://wiki.oroboros.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS O2k-Chamber cleaning with HCl] by removing the glass chamber from the O2k and using a strong acid. | ||
Revision as of 10:42, 12 August 2020
- high-resolution terminology - matching measurements at high-resolution
Biological contamination
Description
Biological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Biological contamination of the O2k-Chamber is detected as a high O2 flux in the closed chamber, measured in a Q1: Oxygen sensor test or QC2: Instrumental O2 background test MiPNet14.06.
- The problem can be solved by first cleaning the O2k-Chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as MiR05. Make sure the 70% ethanol does not contain any additives; e.g., 70 % ethanol used in hospital settings may contain antiseptics (EtOH containing antiseptics provided by Garcia-Roves PM). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under O2k-Chamber cleaning with HCl by removing the glass chamber from the O2k and using a strong acid.
