MiPNet22.04 IOC120 Barcelona ES
 Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry IOC120. »ES Barcelona Zorzano A |
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OROBOROS (2017-03-20) Mitochondr Physiol Network
Abstract:
O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - satellite to MITOEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, ES Barcelona Zorzano A
Programme
| Monday 20th | Topic | Weblink |
| 08:45 | Registration | |
| 09:00-09:15 | Welcome by Prof. Antonio Zorzano. | |
| 09:15-09:30 | Introduction of participants and their research interests. | IOC120 |
| 09:30-10:00 | Get started with the O2k: Overview with video clips. | O2k-Manual |
| 10:00-11:30 | Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay. | The Blue Book - SUIT reference protocol |
| 11:30-12:00 | Coffee/tea break | |
| 12:00-13:00 | Principles of high-resolution respirometry: From switching
on the O2k to the experimental result – oxygen sensor calibration and quality control. |
POS-calibration-SOP |
| 13:00-14:00 | O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol. | O2k-Fluorometry |
| 14:00-15:00 | Lunch | |
| 15:00-17:30 | Demo-Experiment: HRR and O2k-Fluorometry with permeabilized cells – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. | RP1 - RP2 |
| 17:30-18:30 | Protocol driver for Marks and data analysis. | |
| 18:30-20:00 | The Bioblast wiki, O2k-Network and feedback discussion. |
Venue
- Institute for Research in Biomedicine (IRB Barcelona)
- Facultat de Biologia
- Universitat de Barcelona
- c/ Baldiri Reixac, 10-12, 08028
- Barcelona, Spain
General information
- O2k-Fluorometer demo experiment: high-resolution respirometry and H2O2 production (Amplex red)
- Followed by the Barcelona MITOEAGLE Conference, MG and WC Meeting.
Registration
- Please register here for the O2k-Workshop.
- Registration deadline: March 13
Lecturers and tutors
Contact
- OROBOROS INSTRUMENTS
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2017, Next, O2k-Network
