A Lower O2 limit [µM] can be defined in DL protocols for each chamber, to trigger an automatic warning when the experimental O2 concentration declines below this limit as a WARNING to remind the user that re-oxygenation of the medium may be required. In DatLab 7 set the lower O2 limit in the pull-down menu 'Protocol' \ Set DL-Protocol / O2 limit.
- Lower O2 limit [µM]. When checked, an automatic warning notifies the user when the actual oxygen level falls below the specified value for the according chamber: A red flashing "WARNING" button in the status line (DatLab window bottom, right) appears and a "WARNING" event is set in the plot. To reset the actual warning status (to notify the next possible fall-below), the red flashing "WARNING" button has to be clicked. First, a 'Warning window' opens to show all warnings during the experiment. After closing the window, the reset is completed.
- The lower O2 limit should be considered in relation to the critical oxygen concentration, which differs between isolated mitochondria, large cells, and permeabilized muscle fibers.
Keywords: Oxygen signal
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MitoPedia O2k and high-resolution respirometry: DatLab
Labels: MiParea: Instruments;methods