MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver
Abstract: Eigentler A, Draxl A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6.
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
• Keywords: Tissue homogenate, Shredder
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse Tissue;cell: Heart, Nervous system, Liver Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol