Ounpuu 2017 MiP2017
Colorectal cancer cell line (Caco-2) was treated with 1mM sodium butyrate for 48h. The differentiation was determined by alkaline phosphatase (ALP) activity. Mitochondrial respiration was measured by high-resolution respirometry in permeabilized cells. In addition, the enzymatical activities of three main enzymes involved in the transport of energy-rich phosphoryl (AK, CK and hexokinase (HK)) were measured in differentiated and non-differentiated Caco-2 cells.
Treatment of Caco-2 cells with butyrate was accompanied by reduced proliferation and a 7-fold increase in ALP activity. As observed by confocal microscopy, treated cells displayed a tendency to grow in a monolayer and were more elongated compared to cells cultured in the absence of butyrate. Although the mitochondrial respiration remained unaltered, differentiated cells had a more fragmented mitochondrial network with smaller mitochondrial clusters. The activity of HK was slightly increased in differentiated cells, which may indicate an increased level of glycolysis. Treatment of cells with butyrate also resulted in significantly increased CK activity. On the contrary, the total activity of AK was reduced due to decreased AK1 activity.
In conclusion, treatment with 1mM butyrate caused a fragmentation of the mitochondrial network during the differentiation process of Caco2 cells and reestablished CK/AK homeostasis similar to normal large intestine tissue.
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, mt-Medicine, Pharmacology;toxicology Pathology: Cancer
Preparation: Permeabilized cells
- National Inst Chemical Physics Biophysics, Tallinn, Estonia. – email@example.com
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