Pinho 2011 Abstract IOC65
|Pinho JPC, Goncalves RL, Oliveira MF (2011) Studies on the dynamic changes of mitochondrial function following a blood meal in the kissing bug Rhodnius prolixus. MiPNet16.03.|
The physiological implications of hematophagy in arthropods raise interest because many of these organisms are vectors of important diseases. Evidence shows that blood-feeding organisms developed adaptive mechanisms to avoid the toxic products of blood digestion. Since oxidative metabolism is an important source of reactive oxygen species (ROS), we investigated here the hypothesis that the insect Rhodnius prolixus would modulate its mitochondrial function after a blood meal (ABM). We have employed respirometry and spectrofluorimetry to assess oxygen consumption and hydrogen peroxide generation in adult females of R. prolixus flight muscle, respectively. We observed that insects decreased both respiration and hydrogen peroxide (H2O2) production in several mitochondrial metabolic states ABM. The activities of complexes I-III and IV were not distinct ABM, indicating that respiratory chain was not involved on the reduced respiration rates observed. Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an event that was parallel to blood digestion, being most intense 7 days ABM. We also observed a decrease in 78% and 65% in ADP-induced and uncoupled respiration, respectively, throughout the insect life-span. We conclude that blood feeding plays a major role in regulating mitochondrial function and may represent an important adaptation of this insect to hematophagy.
• Keywords: 'Rhodnius prolixus', hematophagy, ROS
• O2k-Network Lab: BR Rio de Janeiro Oliveira MF
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, mt-Medicine
Stress:Oxidative stress;RONS Organism: Hexapods
Preparation: Isolated mitochondria, Enzyme Enzyme: Complex I, Complex III, Complex IV;cytochrome c oxidase, TCA cycle and matrix dehydrogenases Regulation: Inhibitor, Redox state, Substrate Coupling state: OXPHOS