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Anand 2016 PLOS ONE
Additional label 2016-09  +
Coupling states LEAK  + , ROUTINE  + , OXPHOS  + , ET  +
Enzyme Supercomplex  +
Has abstract Mitochondrial cristae are connected to the
Mitochondrial cristae are connected to the inner boundary membrane via crista junctions which are implicated in the regulation of oxidative phosphorylation, apoptosis, and import of lipids and proteins. The MICOS complex determines formation of crista junctions. We performed complexome profiling and identified Mic13, also termed Qil1, as a subunit of the MICOS complex. We show that MIC13 is an inner membrane protein physically interacting with MIC60, a central subunit of the MICOS complex. Using the CRISPR/Cas method we generated the first cell line deleted for MIC13. These knockout cells show a complete loss of crista junctions demonstrating that MIC13 is strictly required for the formation of crista junctions. MIC13 is required for the assembly of MIC10, MIC26, and MIC27 into the MICOS complex. However, it is not needed for the formation of the MIC60/MIC19/MIC25 subcomplex suggesting that the latter is not sufficient for crista junction formation. MIC13 is also dispensable for assembly of respiratory chain complexes and for maintaining mitochondrial network morphology. Still, lack of MIC13 resulted in a moderate reduction of mitochondrial respiration. In summary, we show that MIC13 has a fundamental role in crista junction formation and that assembly of respiratory chain supercomplexes is independent of mitochondrial cristae shape.
ndependent of mitochondrial cristae shape.  +
Has info [ PMID: 27479602 Open Access]  +
Has title Anand R, Strecker V, Urbach J, Wittig I, Reichert AS (2016) Mic13 is essential for formation of crista junctions in mammalian cells. PLOS ONE 11:e0160258.  +
Instrument and method Oxygraph-2k  +
MiP area Respiration  + , mt-Structure;fission;fusion  + , Genetic knockout;overexpression  +
Pathways N  + , CIV  + , NS  + , Other combinations  + , ROX  +
Preparation Permeabilized cells  + , Intact cells  +
Tissue and cell HEK  +
Was published by MiPNetLab DE Frankfurt Reichert A +
Was published in journal PLOS ONE +
Was published in year 2016  +
Was written by Anand R + , Strecker V + , Urbach J + , Wittig I + , Reichert AS +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
14:45:12, 9 November 2017  +
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