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Anmann 2014 Biochim Biophys Acta
Coupling states LEAK  + , OXPHOS  +
Has abstract Adult cardiomyocytes have highly organized
Adult cardiomyocytes have highly organized intracellular structure and energy metabolism whose formation during postnatal development is still largely unclear. Our previous results together with the data from the literature suggest that cytoskeletal proteins, particularly βII-tubulin, are involved in the formation of complexes between mitochondria and energy consumption sites. The aim of this study was to examine the arrangement of intracellular architecture parallel to the alterations in regulation of mitochondrial respiration in rat cardiomyocytes during postnatal development, from 1day to 6months. Respirometric measurements were performed to study the developmental alterations of mitochondrial function. Changes in the mitochondrial arrangement and cytoarchitecture of βII- and αIV-tubulin were examined by confocal microscopy. Our results show that functional maturation of oxidative phosphorylation in mitochondria is completed much earlier than efficient feedback regulation is established between mitochondria and ATPases via creatine kinase system. These changes are accompanied by significant remodeling of regular intermyofibrillar mitochondrial arrays aligned along the bundles of βII-tubulin. Additionally, we demonstrate that formation of regular arrangement of mitochondria is not sufficient per se to provide adult-like efficiency in metabolic feed-back regulation, but organized tubulin networks and reduction in mitochondrial outer membrane permeability for ADP are necessary as well. In conclusion, cardiomyocytes in rat heart become mature on the level of intracellular architecture and energy metabolism at the age of 3months.
d energy metabolism at the age of 3months.  +
Has info [ PMID: 24704335]  +
Has publicationkeywords Mitochondria  + , Bioenergetics  + , Cardiomyocyte development  + , Tubulin  + , Intracellular architecture  +
Has title Anmann T, Varikmaa M, Timohhina N, Tepp K,
Anmann T, Varikmaa M, Timohhina N, Tepp K, Shevchuk I, Chekulayev V, Saks V, Kaambre T (2014) Formation of highly organized intracellular structure and energy metabolism in cardiac muscle cells during postnatal development of rat heart. Biochim Biophys Acta 1837:1350-61.
heart. Biochim Biophys Acta 1837:1350-61.  +
Instrument and method Oxygraph-2k  +
Mammal and model Rat  +
MiP area Respiration  +
Pathways N  + , S  +
Preparation Permeabilized tissue  +
Respiration and regulation ADP  +
Tissue and cell Heart  +
Was published by MiPNetLab EE Tallinn Kaambre T + , EE Tallinn Saks VA +
Was published in journal Biochim Biophys Acta +
Was published in year 2014  +
Was written by Grellier T + , Varikmaa M + , Timohhina N + , Tepp K + , Shevchuk I + , Chekulayev V + , Saks VA + , Kaambre T +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
13:27:27, 7 November 2016  +
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