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Bertolini 2019 MBio
Additional label Labels  + , 2019-05  +
Coupling states LEAK  + , OXPHOS  + , ET  +
Diseases Infectious  +
Has abstract The mitochondrial Ca<sup>2+</sup&
The mitochondrial Ca<sup>2+</sup> uptake in trypanosomatids, which belong to the eukaryotic supergroup Excavata, shares biochemical characteristics with that of animals, which, together with fungi, belong to the supergroup Opisthokonta. However, the composition of the mitochondrial calcium uniporter (MCU) complex in trypanosomatids is quite peculiar, suggesting lineage-specific adaptations. In this work, we used ''Trypanosoma cruzi'' to study the role of orthologs for mitochondrial calcium uptake 1 (MICU1) and MICU2 in mitochondrial Ca<sup>2+</sup> uptake. ''T. cruzi'' MICU1 (TcMICU1) and TcMICU2 have mitochondrial targeting signals, two canonical EF-hand calcium-binding domains, and localize to the mitochondria. Using the CRISPR/Cas9 system (i.e., clustered regularly interspaced short palindromic repeats with Cas9), we generated TcMICU1 and TcMICU2 knockout (-KO) cell lines. Ablation of either TcMICU1 or TcMICU2 showed a significantly reduced mitochondrial Ca<sup>2+</sup> uptake in permeabilized epimastigotes without dissipation of the mitochondrial membrane potential or effects on the AMP/ATP ratio or citrate synthase activity. However, none of these proteins had a gatekeeper function at low cytosolic Ca<sup>2+</sup> concentrations ([Ca<sup>2+</sup>]<sub>cyt</sub>), as occurs with their mammalian orthologs. TcMICU1-KO and TcMICU2-KO epimastigotes had a lower growth rate and impaired oxidative metabolism, while infective trypomastigotes have a reduced capacity to invade host cells and to replicate within them as amastigotes. The findings of this work, which is the first to study the role of MICU1 and MICU2 in organisms evolutionarily distant from animals, suggest that, although these components were probably present in the last eukaryotic common ancestor (LECA), they developed different roles during evolution of different eukaryotic supergroups. The work also provides new insights into the adaptations of trypanosomatids to their particular life styles. ''Trypanosoma cruzi'' is the etiologic agent of Chagas disease and belongs to the early-branching eukaryotic supergroup Excavata. Its mitochondrial calcium uniporter (MCU) subunit shares similarity with the animal ortholog that was important to discover its encoding gene. In animal cells, the MICU1 and MICU2 proteins act as Ca<sup>2+</sup> sensors and gatekeepers of the MCU, preventing Ca<sup>2+</sup> uptake under resting conditions and favoring it at high cytosolic Ca<sup>2+</sup> concentrations ([Ca<sup>2+</sup>]<sub>cyt</sub>). Using the CRISPR/Cas9 technique, we generated TcMICU1 and TcMICU2 knockout cell lines and showed that MICU1 and -2 do not act as gatekeepers at low [Ca<sup>2+</sup>]<sub>cyt</sub> but are essential for normal growth, host cell invasion, and intracellular replication, revealing lineage-specific adaptations. <small>Copyright © 2019 Bertolini et al.</small>
ight © 2019 Bertolini et al.</small>  +
Has editor [[Plangger M]]  +
Has info [https://www.ncbi.nlm.nih.gov/pubmed/31064825 PMID: 31064825 Open Access]  +
Has publicationkeywords MICU1  + , MICU2  + , Trypanosoma cruzi  + , Calcium signaling  + , Calcium uniporter  + , Mitochondria  +
Has title Bertolini MS, Chiurillo MA, Lander N, Vercesi AE, Docampo R (2019) MICU1 and MICU2 play an essential role in mitochondrial Ca<sup>2+</sup> uptake, growth, and infectivity of the human pathogen ''Trypanosoma cruzi''. MBio 10:e00348-19.  +
Instrument and method Oxygraph-2k  +
Mammal and model Protists  +
MiP area Respiration  + , Genetic knockout;overexpression  +
Preparation Permeabilized cells  +
Respiration and regulation Ion;substrate transport  +
Was published by MiPNetLab BR Campinas Vercesi AE +
Was published in journal MBio +
Was published in year 2019  +
Was written by Bertolini MS + , Chiurillo MA + , Lander N + , Vercesi AE + , Docampo R +
Categories Publications
Modification date
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12:52:05, 14 May 2019  +
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