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Lanza 2013 Am J Physiol Endocrinol Metab
Coupling states LEAK  + , OXPHOS  +
Has abstract Omega-3 polyunsaturated fatty acids (n-3 P
Omega-3 polyunsaturated fatty acids (n-3 PUFAs) enhance insulin sensitivity and glucose homeostasis in rodent models of insulin resistance. These beneficial effects have been linked with anti-inflammatory properties, but emerging data suggest that the mechanisms may also converge on mitochondria. We evaluated the influence of dietary n-3 PUFAs on mitochondrial physiology and muscle lipid metabolites in the context of high-fat diet (HFD) in mice. Mice were fed control diets (10% fat), HFD (60% fat), or HFD with fish oil (HFD+FO, 3.4% kcal from n-3 PUFAs) for 10 wk. Body mass and fat mass increased similarly in HFD and HFD+FO, but n-3 PUFAs attenuated the glucose intolerance that developed with HFD and increased expression of genes that regulate glucose metabolism in skeletal muscle. Despite similar muscle triglyceride levels in HFD and HFD+FO, long-chain acyl-CoAs and ceramides were lower in the presence of fish oil. Mitochondrial abundance and oxidative capacity were similarly increased in HFD and HFD+FO compared with controls. Hydrogen peroxide production was similarly elevated in HFD and HFD+FO in isolated mitochondria but not in permeabilized muscle fibers, likely due to increased activity and expression of catalase. These results support a hypothesis that n-3 PUFAs protect glucose tolerance, in part by preventing the accumulation of bioactive lipid mediators that interfere with insulin action. Furthermore, the respiratory function of skeletal muscle mitochondria does not appear to be a major factor in sphingolipid accumulation, glucose intolerance, or the protective effects of n-3 PUFAs.
e, or the protective effects of n-3 PUFAs.  +
Has info [ PMID: 23632634 Open Access]  +
Has publicationkeywords Amplex Red in muscle fibers  + , Buffer z  + , Ceramide  + , Essential fatty acids  + , Fish oil  + , High-fat diet  + , Insulin sensitivity  + , Long-chain acyl-coenzyme A  + , Mitochondria  + , Omega-3 fatty acids  + , Polyunsaturated fatty acids  + , Sphingolipid  +
Has title Lanza IR, Blachnio-Zabielska A, Johnson ML
Lanza IR, Blachnio-Zabielska A, Johnson ML, Schimke JM, Jakaitis DR, Lebrasseur NK, Jensen MD, Sreekumaran Nair K, Zabielski P (2013) Influence of fish oil on skeletal muscle mitochondrial energetics and lipid metabolites during high-fat diet. Am J Physiol Endocrinol Metab 304:E1391-403.
J Physiol Endocrinol Metab 304:E1391-403.  +
Instrument and method Oxygraph-2k  +
Mammal and model Mouse  +
MiP area Exercise physiology;nutrition;life style  + , Respiration  + , mt-Biogenesis;mt-density  +
Pathways F  + , N  + , NS  + , ROX  + , S  +
Preparation Isolated mitochondria  +
Stress Oxidative stress;RONS  +
Tissue and cell Skeletal muscle  +
Was published by MiPNetLab US MN Rochester Nair KS +
Was published in journal Am J Physiol Endocrinol Metab +
Was published in year 2013  +
Was written by Lanza IR + , Blachnio-Zabielska A + , Johnson ML + , Schimke JM + , Jakaitis DR + , Lebrasseur NK + , Jensen MD + , Sreekumaran Nair K + , Zabielski P +
Categories Publications
Modification date
"Modification date" is a predefined property that corresponds to the date of the last modification of a subject and is provided by Semantic MediaWiki.
09:34:28, 18 April 2018  +
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