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...ormance and data analysis. Therefore, we provide guidelines for performing and evaluating respirometric assays. ...ter-laboratory data harmonization, fighting the [[reproducibility crisis]] in science.

...to the cytoplasm is associated with apoptosis. Cytochrome ''c'' is applied in [[HRR]] to test the integrity of the [[mitochondrial outer membrane]] ([[cy {{MitoPedia O2k and high-resolution respirometry}}

...bstrates, ''R'' depends on endogenous substrates. ''R'' cannot be measured in [[permeabilized cells]] or [[isolated mitochondria]]. ''R'' is corrected fo ...itive P-phase. Proton flow to the negative matrix phase (H⁺_in) drives the phosphorylation of ADP to ATP. Proton leaks dissipate ene

|title=[[Image:O2k-Info.png|right|60px|link=About Oroboros|About Oroboros]]DatLab 7: innovations. |abstract=Doerrier C, Tindle-Solomon L, Gradl L, Gnaiger E (2019) DatLab 7.4: innovations. Mitochondr Physiol Network 21.16(03)

...ity in experimental design by combining the technical advantages of closed and [[open system]]s approaches. == DatLab oxygen flux: performance and data analysis ==

...solar energy (in photosynthetic cells) or chemiosmotic energy (respiration in heterotrophic cells). ADP is added to [[mitochondrial preparations]] at kin {{MitoPedia O2k and high-resolution respirometry}}

...elevated oxygen regime is applied, requiring instrumental background test in the same range of elevated oxygen. ...es]] for how to find the '''Template O2 background.xlsx''' (available with DatLab 7.4 software).

...180px]]'''O2k-pH ISE-Module''': two pH electrodes and reference electrodes and accessories ...://www.oroboros.at/index.php/product/o2k-ph-ise-module/ '''Product details and purchase information''']

...'Tetraphenylphosphonium''' (TPP⁺). A lipophilic molecular probe in conjunction with an ion selective electrode (ISE) for [[Mitochondrial membr == Application in [[HRR]] ==