From Bioblast
Description
Rhodamine 123 is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. Rh123 is a lipophilic cation that is accumulated by mitochondria in proportion to ΞΟmt. Using ethanol as the solvent, the excitation maximum is511 nm and the emission maximum is 534 nm.The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively (Sigma-Aldrich).
Abbreviation: Rh123
Template:Keywords Force and membrane potential
Application in HRFR
Life Technologies, R302, 25 mg, store at -20 Β°C, FW=380.83
- Preparation of 5 mM storage solution (dissolved in ethanol)
- Weight 1.9 mg of Rh123 and dissolve in 1 mL ethanol;
- Divide into 0.1 mL portions (use dark vials);
- Store at -20 Β°C.
- Preparation of 5 mM storage solution (dissolved in ethanol)
- Preparation of 0.2 mM stock solution
- Dilute a 100 Β΅L aliquot 5 mM storage solution with 2400 Β΅L ethanol to reach 2.5 mL final volume;
- Divide into 0.2 mL portions (use dark vials);
- Store at -20 Β°C.
- Preparation of 0.2 mM stock solution
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the Amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
- Output: The focus of the output with Rh123 is on Type C: Force, mt-membrane potential.
Rh123 with the O2k-FluoRespirometer: exploratory experiments
MitoPedia methods:
Fluorometry
Labels: MiParea: Respiration, Instruments;methods
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-MultiSensor