Difference between revisions of "2-Deoxyglucose"
From Bioblast
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|info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | |info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | ||
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== Application in [[HRR]] == | |||
:::'''2-DG: 2-deoxy-D-glucose''' C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>); Sigma D6134, 1 g or 5 g, store at 2-8 °C; FW = 164.16 | |||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# weigh 1g of 2-deoxy-D-glucose | |||
::::# dissolve 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | |||
:::»'''O2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 25 µL using a 50 µL syringe | |||
::::* Final conc. in 2 mL O2k-chamber: 25 mM | |||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
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|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Revision as of 09:57, 11 September 2020
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase .
Abbreviation: 2-DG
Reference: Proton_flux, O2k-pH_ISE-Module
Application in HRR
- 2-DG: 2-deoxy-D-glucose C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 °C; FW = 164.16
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 1g of 2-deoxy-D-glucose
- dissolve 3.0458 mL H2O in a 10 mL-glass beaker
- »O2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 25 µL using a 50 µL syringe
- Final conc. in 2 mL O2k-chamber: 25 mM
MitoPedia topics:
Inhibitor