Difference between revisions of "2-Deoxyglucose"

Latest revision as of 11:16, 21 June 2021

high-resolution terminology - matching measurements at high-resolution

2-Deoxyglucose

Description

2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.

Abbreviation: 2-DG

Reference: Proton_flux, O2k-pH_ISE-Module

Application in HRR

2-DG: 2-deoxy-D-glucose (C₆H₁₂0₅); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol^-1

Preparation of 2 M stock solution (dissolved in H₂O):

weigh 1 g of 2-deoxy-D-glucose
dissolve with 3.0458 mL H₂O in a 10 mL-glass beaker

»O2k manual titrations: MiPNet09.12 O2k-Titrations

Titration volume: 25 µL using a 50 µL syringe
Final conc. in 2 mL O2k-chamber: 25 mM

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=2-DG
-|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase .
+|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
 |info=[[Proton_flux]], [[O2k-pH_ISE-Module]]
 }}
+__TOC__
+== Application in [[HRR]] ==
+:::'''2-DG:  2-deoxy-D-glucose'''   (C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>);  Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol<sup>-1</sup>
+::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
+::::# weigh 1 g of 2-deoxy-D-glucose
+::::# dissolve with 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker
+:::»'''O2k manual titrations:'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume: 25 µL using a 50 µL syringe
+::::* Final conc. in 2 mL O2k-chamber: 25 mM
 {{MitoPedia concepts}}
 {{MitoPedia methods}}
@@ Line 10: / Line 24: @@
 |mitopedia topic=Inhibitor
 }}
-== Application in [[HRR]] ==
-'''2-DG:  2-deoxy-D-glucose'''   C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>);  Sigma D6134, 1 g or 5 g, store at 2-8 °C; FW = 164.16
-'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
-::1) weigh 1g of 2-deoxy-D-glucose
-::2) dissolve 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker
-'''Oxygraph-2k Titration:'''
-::* Titration volume: 25 µL using a 50 µL syringe
-::* Final conc. in 2 mL O2k-chamber: 25 mM