Difference between revisions of "2-Deoxyglucose"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::'''2-DG: 2-deoxy-D-glucose''' C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol<sup>-1</sup> | :::'''2-DG: 2-deoxy-D-glucose''' (C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol<sup>-1</sup> | ||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
::::# weigh | ::::# weigh 1 g of 2-deoxy-D-glucose | ||
::::# dissolve 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | ::::# dissolve with 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | ||
:::»'''O2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | ||
Latest revision as of 11:16, 21 June 2021
- high-resolution terminology - matching measurements at high-resolution
2-Deoxyglucose
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
Abbreviation: 2-DG
Reference: Proton_flux, O2k-pH_ISE-Module
Application in HRR
- 2-DG: 2-deoxy-D-glucose (C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 °C; M = 164.16 g·mol-1
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 1 g of 2-deoxy-D-glucose
- dissolve with 3.0458 mL H2O in a 10 mL-glass beaker
- »O2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 25 µL using a 50 µL syringe
- Final conc. in 2 mL O2k-chamber: 25 mM
MitoPedia topics:
Inhibitor
