Blebbistatin: Difference between revisions
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|description=Blebbistatin is a | |description=Blebbistatin is a widely used muscle and non-muscle myosin II-specific inhibitor that block contractile activity. Blebbistatin shows selectivity and high affinity for multiple class II myosins. Blebbistatin is commonly employed in respirometric experiments with permeabilized muscle fibers (pfi). Permeabilized muscle fibers are sensitive to low oxygen supply due to diffusion restrictions that limit mitochondrial respiration at the core of the fiber bundle. Therefore, hyperoxic conditions are required to counteract this limitation. Further studies have shown that the addition of blebbistatin in the respiration medium prevents fiber contraction, reduces the oxygen sensitivity and allows the study of ADP kinetics in pfi at normoxic oxygen levels. However, other studies described that the presence of blebbistatin does not prevent the oxygen dependence in pfi. Several limitations of blebbistatin i.e. low solubility in water, cytotoxicity and phototoxicity have been described. | ||
|info=[[Perry 2011 Biochem J]] | |info=[[Perry 2011 Biochem J]] | ||
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|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
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== References == | == References: Blebbistatin == | ||
{{#ask:[[Category:Publications]] [[Additional label::Blebbistatin]] | {{#ask:[[Category:Publications]] [[Additional label::Blebbistatin]] | ||
|?Was published in year=Year | |?Was published in year=Year | ||
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:::# Kovács M, Tóth J, Hetényi C, Málnási-Csizmadia A, Sellers JR (2004) Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34):35557-63. - https://www.ncbi.nlm.nih.gov/pubmed/15205456 | :::# Kovács M, Tóth J, Hetényi C, Málnási-Csizmadia A, Sellers JR (2004) Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34):35557-63. - https://www.ncbi.nlm.nih.gov/pubmed/15205456 | ||
:::# [[Scandurra 2010 Adv Exp Med Biol]] | :::# Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25 - [[Scandurra 2010 Adv Exp Med Biol]] | ||
:::# Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A (2016) A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep 6:26141 - https://www.ncbi.nlm.nih.gov/pubmed/27241904 | :::# Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A (2016) A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep 6:26141 - https://www.ncbi.nlm.nih.gov/pubmed/27241904 |
Revision as of 20:33, 8 March 2020
Description
Blebbistatin is a widely used muscle and non-muscle myosin II-specific inhibitor that block contractile activity. Blebbistatin shows selectivity and high affinity for multiple class II myosins. Blebbistatin is commonly employed in respirometric experiments with permeabilized muscle fibers (pfi). Permeabilized muscle fibers are sensitive to low oxygen supply due to diffusion restrictions that limit mitochondrial respiration at the core of the fiber bundle. Therefore, hyperoxic conditions are required to counteract this limitation. Further studies have shown that the addition of blebbistatin in the respiration medium prevents fiber contraction, reduces the oxygen sensitivity and allows the study of ADP kinetics in pfi at normoxic oxygen levels. However, other studies described that the presence of blebbistatin does not prevent the oxygen dependence in pfi. Several limitations of blebbistatin i.e. low solubility in water, cytotoxicity and phototoxicity have been described.
Reference: Perry 2011 Biochem J
MitoPedia concepts:
MiP concept
MitoPedia topics:
Inhibitor
References: Blebbistatin
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2019 MitoFit Preprint Arch EA | 2019 | Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0009 | Human | Skeletal muscle |
Perry 2011 Biochem J | 2011 | Perry CG, Kane DA, Lin CT, Kozy R, Cathey BL, Lark DS, Kane CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437:215-22. | Human Rat | Skeletal muscle |
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2019 MiP2019 | 2019 | Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. | Human | Skeletal muscle |
Doerrier 2018 MiP2018a | 2018 | Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers. | Mouse | Skeletal muscle |
Bezuidenhout 2016a Abstract MitoFit Science Camp 2016 | 2016 | Comparison of oxygen dependence of respiration in permeabilized mouse skeletal muscle fibers in two respiration media, MiR06Cr and Buffer Z containing Ctl, Cr and Blebbistatin. | Mouse | Skeletal muscle |
- Kovács M, Tóth J, Hetényi C, Málnási-Csizmadia A, Sellers JR (2004) Mechanism of blebbistatin inhibition of myosin II. J Biol Chem 279(34):35557-63. - https://www.ncbi.nlm.nih.gov/pubmed/15205456
- Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25 - Scandurra 2010 Adv Exp Med Biol
- Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A (2016) A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep 6:26141 - https://www.ncbi.nlm.nih.gov/pubmed/27241904