Difference between revisions of "Calcium Green"

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Calcium Green

Description

Calcium green denotes a family of extrinsic fluorophores applied for measurement of Ca²⁺ concentration.

Abbreviation: CaG

MitoPedia methods: Fluorometry

Calcium green^TM is a registered trademark and available from Life Technologies (former Invitrogen) in several formulations. All fluorophores of the Calcium green family have the same fluorophoric moiety in their molecular structure and therefore near identical optical properties. They differ in their K_D values and are suitable for measuring different ranges of calcium concentrations.

Calcium green 1: K_D = 190 nM
Calcium green-2: K_D = 550 nM
Calcium green-5N: K_D = 14000 nM

Measurement: See Calcium for method independent considerations related to measuring calcium levels, covering e.g.

important consideration before starting a project involving calcium measurements
Ca2+ buffering
calculation of free Ca2+ concentrations
Ca2+ calibration buffers.

See O2k-Fluorescence LED2-Module for specific aspects of fluorescence measurements using the O2k. On the fluorophores producers homepage detailed product information (| Long-Wavelength Calcium Indicators Product Information , |CalciumCalibration) as well as general information on measuring calcium (e.g. [1], [2]) is available.

The three Calcium green fluorophores and Magnesium green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.

Use of Calcium green-5N with the O2k-Fluorescence LED2-Module: Elustondo 2014 Biochim Biophys Acta
Anderson_2011_Am_J_Physiol_Heart_Circ_Physiol

@@ Line 14: / Line 14: @@
-'''Measurement:''' See [[Calcium]] for method independent considerations about measuring calcium levels, covering e.g. important consideration before starting a project involving calcium measurements, Ca2+ buffering, calculation of free Ca2+ concentrations, and  Ca2+ calibration buffers. See [[O2k-Fluorescence LED2-Module]] for specific aspects of fluorescence measurements using the O2k. On the fluorophores producers homepage detailed product information ([https://tools.lifetechnologies.com/content/sfs/manuals/mp03010.pdf | Long-Wavelength Calcium Indicators Product Information ], [http://tools.lifetechnologies.com/content/sfs/manuals/CalciumCalibrationBufferKits_PI.pdf |CalciumCalibration]) as well as general information on measuring calcium (e.g. [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/introduction-to-ca2-measurements-with-fluorescent-indicators.html], [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-ca2-indicators-excited-with-visible-light.html]) is available.
+'''Measurement:''' See [[Calcium]] for method independent considerations related to measuring calcium levels, covering e.g.
+* important consideration before starting a project involving calcium measurements
+* Ca2+ buffering
+* calculation of free Ca2+ concentrations
+* Ca2+ calibration buffers.
+See [[O2k-Fluorescence LED2-Module]] for specific aspects of fluorescence measurements using the O2k.
+On the fluorophores producers homepage detailed product information ([https://tools.lifetechnologies.com/content/sfs/manuals/mp03010.pdf | Long-Wavelength Calcium Indicators Product Information ], [http://tools.lifetechnologies.com/content/sfs/manuals/CalciumCalibrationBufferKits_PI.pdf |CalciumCalibration]) as well as general information on measuring calcium (e.g. [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/introduction-to-ca2-measurements-with-fluorescent-indicators.html], [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-ca2-indicators-excited-with-visible-light.html]) is available.
 The three Calcium green fluorophores and [[Magnesium green]] share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same [[Fluorescence-Sensor Blue|fluorescence sensor]] and [[Filter Set MgG / CaG|filter set]] can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.