Catalase

high-resolution terminology - matching measurements at high-resolution

Catalase

Description

Catalase catalyzes the dismutation of hydrogen peroxide to water and oxygen.

Abbreviation: Ctl

Reference: MiPNet09.12

MitoPedia topics: Enzyme 

Application in HRR

Clt: Catalase (from bovine liver,lyophilized powder, 2.000-5.000 units/mg protein); Sigma C 9322, store at -20 °C

Catalase is included in the mitochondrial respiration medium MiR06 (MiR06 = MiR05 plus catalase), so as to allow for re-oxygenation of the respiration medium (without lifting the stopper and risking the introduction of air bubbles) by addition of a small volume of a 200 mM stock solution of H₂O₂.

Preparation of catalase stock solution

Stock solution: 112.000 u/ml (dissolved in MiR05)

Example: 'Catalase lypophilized powder, 2800 units/mg solid and 3500 units/mg protein'

1) Use 'units/mg solid' for your calculations

2) Result: 40 mg catalase powder (2800 U/mg) are dissolved in 1 ml MiR05 to obtain a catalase stock solution with 112.000 U/ml.

Unit definition: * Units of enzymatic activitiy (u) in µmol/min; assay used by Sigma Aldrich: ' One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀. '

Oxygraph-2k manual titrations: MiPNet09.12

• Titration volume: 5 µl Ctl using a 25 µl syringe
• Final conc. in 2 ml O2k-chamber: 280 u/ml