Difference between revisions of "Chung 2018 J Exp Biol"

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Chung DJ, Sparagna GC, Chicco AJ, Schulte PM (2018) Patterns of mitochondrial membrane remodeling parallel functional adaptations to thermal stress. J Exp Biol 221. pii: jeb174458.

» PMID: 29643174 Open Access

Chung DJ, Sparagna GC, Chicco AJ, Schulte PM (2018) J Exp Biol

Abstract: The effect of temperature on mitochondrial performance is thought to be partly due to its effect on mitochondrial membranes. Numerous studies have shown that thermal acclimation and adaptation can alter the amount of inner-mitochondrial membrane (mtIM), but little is known about the capacity of organisms to modulate mitochondrial membrane composition. Using northern and southern subspecies of Atlantic killifish (Fundulus heteroclitus) that are locally adapted to different environmental temperatures, we assessed whether thermal acclimation altered liver mitochondrial respiratory capacity or the composition and amount of mtIM. We measured changes in phospholipid headgroups and headgroup-specific fatty acid (FA) remodeling, and used respirometry to assess mitochondrial respiratory capacity. Acclimation to 5 °C and 33 °C altered mitochondrial respiratory capacity in both subspecies. Northern F. heteroclitus exhibited greater mitochondrial respiratory capacity across acclimation temperatures, consistent with previously observed subspecies differences in whole-organism aerobic metabolism. Mitochondrial phospholipids were altered following thermal acclimation, and the direction of these changes was largely consistent between subspecies. These effects were primarily driven by remodeling of specific phospholipid classes and were associated with shifts in metabolic phenotypes. There were also differences in membrane composition between subspecies that were driven largely by differences in phospholipid classes. Changes in respiratory capacity between subspecies and with acclimation were largely but not completely accounted for by alterations in the amount of mtIM. Taken together, these results support a role for changes in liver mitochondrial function in the ectothermic response to thermal stress during both acclimation and adaptation, and implicate lipid remodeling as a mechanism contributing to these changes. • Keywords: Acclimation, Fatty acid, Killifish, Phospholipid, Temperature • Bioblast editor: Kandolf G • O2k-Network Lab: CA Vancouver Richards JG, US CO Aurora Sparagna GC, US CO Fort Collins Chicco AJ

Post-peer review: added reference

Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8

Labels: MiParea: Respiration, mt-Membrane

Stress:Temperature Organism: Fishes Tissue;cell: Liver Preparation: Isolated mitochondria

Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, CIV, NS, ROX HRR: Oxygraph-2k

Labels, 2018-05

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 {{Publication
 |title=Chung DJ, Sparagna GC, Chicco AJ, Schulte PM (2018) Patterns of mitochondrial membrane remodeling parallel functional adaptations to thermal stress. J Exp Biol 221. pii: jeb174458.
-|info=[https://www.ncbi.nlm.nih.gov/pubmed/29643174 PMID: 29643174]
+|info=[https://www.ncbi.nlm.nih.gov/pubmed/29643174 PMID: 29643174 Open Access]
 |authors=Chung DJ, Sparagna GC, Chicco AJ, Schulte PM
 |year=2018
 |journal=J Exp Biol
-|abstract=The effect of temperature on mitochondrial performance is thought to be partly due to its effect on mitochondrial membranes. Numerous studies have shown that thermal acclimation and adaptation can alter the amount of inner-mitochondrial membrane (IMM), but little is known about the capacity of organisms to modulate mitochondrial membrane composition. Using northern and southern subspecies of Atlantic killifish (''Fundulus heteroclitus'') that are locally adapted to different environmental temperatures, we assessed whether thermal acclimation altered liver mitochondrial respiratory capacity or the composition and amount of IMM. We measured changes in phospholipid headgroups and headgroup-specific fatty acid (FA) remodeling, and used respirometry to assess mitochondrial respiratory capacity. Acclimation to 5°C and 33°C altered mitochondrial respiratory capacity in both subspecies. Northern ''F. heteroclitus'' exhibited greater mitochondrial respiratory capacity across acclimation temperatures, consistent with previously observed subspecies differences in whole-organism aerobic metabolism. Mitochondrial phospholipids were altered following thermal acclimation, and the direction of these changes was largely consistent between subspecies. These effects were primarily driven by remodeling of specific phospholipid classes and were associated with shifts in metabolic phenotypes. There were also differences in membrane composition between subspecies that were driven largely by differences in phospholipid classes. Changes in respiratory capacity between subspecies and with acclimation were largely but not completely accounted for by alterations in the amount of IMM. Taken together, these results support a role for changes in liver mitochondrial function in the ectothermic response to thermal stress during both acclimation and adaptation, and implicate lipid remodeling as a mechanism contributing to these changes.
+|abstract=The effect of temperature on mitochondrial performance is thought to be partly due to its effect on mitochondrial membranes. Numerous studies have shown that thermal acclimation and adaptation can alter the amount of inner-mitochondrial membrane (mtIM), but little is known about the capacity of organisms to modulate mitochondrial membrane composition. Using northern and southern subspecies of Atlantic killifish (''Fundulus heteroclitus'') that are locally adapted to different environmental temperatures, we assessed whether thermal acclimation altered liver mitochondrial respiratory capacity or the composition and amount of mtIM. We measured changes in phospholipid headgroups and headgroup-specific fatty acid (FA) remodeling, and used respirometry to assess mitochondrial respiratory capacity. Acclimation to 5 °C and 33 °C altered mitochondrial respiratory capacity in both subspecies. Northern ''F. heteroclitus'' exhibited greater mitochondrial respiratory capacity across acclimation temperatures, consistent with previously observed subspecies differences in whole-organism aerobic metabolism. Mitochondrial phospholipids were altered following thermal acclimation, and the direction of these changes was largely consistent between subspecies. These effects were primarily driven by remodeling of specific phospholipid classes and were associated with shifts in metabolic phenotypes. There were also differences in membrane composition between subspecies that were driven largely by differences in phospholipid classes. Changes in respiratory capacity between subspecies and with acclimation were largely but not completely accounted for by alterations in the amount of mtIM. Taken together, these results support a role for changes in liver mitochondrial function in the ectothermic response to thermal stress during both acclimation and adaptation, and implicate lipid remodeling as a mechanism contributing to these changes.
 |keywords=Acclimation, Fatty acid, Killifish, Phospholipid, Temperature
 |editor=[[Kandolf G]]
 |mipnetlab=CA Vancouver Richards JG, US CO Aurora Sparagna GC, US CO Fort Collins Chicco AJ
 }}
+== Post-peer review: added reference ==
+::::* Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep |doi:10.1038/s41598-017-02789-8]]
 {{Labeling
 |area=Respiration, mt-Membrane