Difference between revisions of "Dinitrophenole"
From Bioblast
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|type=Uncoupler | |type=Uncoupler | ||
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'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ||
<span style="color:#8B008B"> '''Caution:''' Toxic!</span> | :::: <span style="color:#8B008B"> '''Caution:''' Toxic!</span> | ||
:: | :::# Weigh 3.7 mg of DNP (FW = 184.11). | ||
:: | :::# Dissolve in 1.2 ml H<sub>2</sub>O. | ||
:: | :::# Neutralize with 1 N KOH. | ||
:: | :::# Adjust final volume to 2 ml and divide into 0.2 ml portions. | ||
:: | :::# Store at -20 Β°C. |
Revision as of 08:51, 20 March 2016
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
MitoPedia topics: Uncoupler
- Preparation of 10 mM DNP stock solution (dissolved in H2O):
- Caution: Toxic!
- Weigh 3.7 mg of DNP (FW = 184.11).
- Dissolve in 1.2 ml H2O.
- Neutralize with 1 N KOH.
- Adjust final volume to 2 ml and divide into 0.2 ml portions.
- Store at -20 Β°C.