Difference between revisions of "Dinitrophenole"
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2,4-dinitrophenol (DNP) is a compound design for the manufacture of ammunitions during the first world war and lately described in 1933 by Maurice Tainter as a weight loss drug due to its effect into the besal metabolic rate. DNP decreases the formation of high-energy phosphate bonds in mitochondria preventing the uptake of inorganic phosphate molecules into the mitochondria. At the same time, its characteristics as ionophore stimulates systemic oxygen consumption, increasing the basal leak of protons. This shift in the proton electrochemical gradient then results in potential energy dissipating as heat, instead of being converted to ATP, leading to uncontrolled hyperthermia. | |||
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|mitopedia topic=Uncoupler | |mitopedia topic=Uncoupler | ||
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== Application in [[HRFR]] == | |||
::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ||
Revision as of 11:47, 10 September 2018
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
2,4-dinitrophenol (DNP) is a compound design for the manufacture of ammunitions during the first world war and lately described in 1933 by Maurice Tainter as a weight loss drug due to its effect into the besal metabolic rate. DNP decreases the formation of high-energy phosphate bonds in mitochondria preventing the uptake of inorganic phosphate molecules into the mitochondria. At the same time, its characteristics as ionophore stimulates systemic oxygen consumption, increasing the basal leak of protons. This shift in the proton electrochemical gradient then results in potential energy dissipating as heat, instead of being converted to ATP, leading to uncontrolled hyperthermia.
MitoPedia topics: Uncoupler
Application in HRFR
- Preparation of 10 mM DNP stock solution (dissolved in H2O):
- Caution: Toxic!
- Weigh 3.7 mg of DNP (FW = 184.11).
- Dissolve in 1.2 ml H2O.
- Neutralize with 1 M KOH.
- Adjust final volume to 2 ml and divide into 0.2 ml portions.
- Store at -20 °C.
