Difference between revisions of "Dinitrophenole"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=DNP | |abbr=DNP | ||
|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184 | |description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a proton ionophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]]. | ||
|info=[[SteinlechnerMaran_1996_AJP]] | |info=[[SteinlechnerMaran_1996_AJP]] | ||
|type=Uncoupler | |type=Uncoupler | ||
| Line 9: | Line 9: | ||
|type=Uncoupler | |type=Uncoupler | ||
}} | }} | ||
'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | |||
<span style="color:#8B008B"> '''Caution:''' Toxic!.</span> | |||
::1) weigh 3.7 mg of DNP (FW = 184.11) | |||
::2) dissolve in 1.2 ml H2O | |||
::3) neutralize with 1 N KOH | |||
::4) adjust final volume to 2 ml and divide into 0.2 ml portions | |||
::6) store frozen at -20 °C | |||
Revision as of 11:19, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a proton ionophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: SteinlechnerMaran_1996_AJP
MitoPedia topics:
Uncoupler
Preparation of 10 mM DNP stock solution (dissolved in H2O):
Caution: Toxic!.
- 1) weigh 3.7 mg of DNP (FW = 184.11)
- 2) dissolve in 1.2 ml H2O
- 3) neutralize with 1 N KOH
- 4) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -20 °C
