Difference between revisions of "Dinitrophenole"
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'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | ||
<span style="color:#8B008B"> '''Caution:''' Toxic! | <span style="color:#8B008B"> '''Caution:''' Toxic!</span> | ||
::1) weigh 3.7 mg of DNP (FW = 184.11) | ::1) weigh 3.7 mg of DNP (FW = 184.11) | ||
Revision as of 11:20, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a proton ionophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: SteinlechnerMaran_1996_AJP
MitoPedia topics:
Uncoupler
Preparation of 10 mM DNP stock solution (dissolved in H2O):
Caution: Toxic!
- 1) weigh 3.7 mg of DNP (FW = 184.11)
- 2) dissolve in 1.2 ml H2O
- 3) neutralize with 1 N KOH
- 4) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -20 °C
