Difference between revisions of "Dinitrophenole"

Revision as of 11:34, 18 May 2012

high-resolution terminology - matching measurements at high-resolution

Dinitrophenole

2,4-dinitrophenol (C₆H₄N₂O₅; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.

Abbreviation: DNP

Reference: SteinlechnerMaran_1996_AJP

MitoPedia topics: Uncoupler

Preparation of 10 mM DNP stock solution (dissolved in H₂O):

Caution: Toxic!

1) weigh 3.7 mg of DNP (FW = 184.11)

2) dissolve in 1.2 ml H2O

3) neutralize with 1 N KOH

4) adjust final volume to 2 ml and divide into 0.2 ml portions

6) store frozen at -20 °C

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 {{MitoPedia
 |abbr=DNP
-|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a proton ionophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]].
+|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]].
 |info=[[SteinlechnerMaran_1996_AJP]]
 |type=Uncoupler