Difference between revisions of "Dinitrophenole"

Revision as of 21:35, 21 April 2014

high-resolution terminology - matching measurements at high-resolution

Dinitrophenole

2,4-dinitrophenol (C₆H₄N₂O₅; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.

Abbreviation: DNP

Reference: SteinlechnerMaran_1996_AJP

MitoPedia topics: Uncoupler

Preparation of 10 mM DNP stock solution (dissolved in H₂O):

Caution: Toxic!

1. Weigh 3.7 mg of DNP (FW = 184.11).

2. Dissolve in 1.2 ml H2O.

3. Neutralize with 1 N KOH.

4. Adjust final volume to 2 ml and divide into 0.2 ml portions.

6. Store at -20 °C.

@@ Line 4: / Line 4: @@
 |info=[[SteinlechnerMaran_1996_AJP]]
 |type=Uncoupler
+}}
+{{MitoPedia methods|type=Uncoupler
 }}
 {{MitoPedia topics
@@ Line 9: / Line 11: @@
 |type=Uncoupler
 }}
 '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):
 <span style="color:#8B008B"> '''Caution:''' Toxic!</span>
-::1) weigh 3.7 mg of DNP (FW = 184.11)
+::1. Weigh 3.7 mg of DNP (FW = 184.11).
-::2) dissolve in 1.2 ml H2O
+::2. Dissolve in 1.2 ml H2O.
-::3) neutralize with 1 N KOH
+::3. Neutralize with 1 N KOH.
-::4) adjust final volume to 2 ml and divide into 0.2 ml portions
+::4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
-::6) store frozen at -20 °C
+::6. Store at -20 °C.