Difference between revisions of "Dinitrophenole"
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|abbr=DNP | |abbr=DNP | ||
|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]]. | |description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]]. | ||
|info=[[ | |info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]] | ||
|type=Uncoupler | |type=Uncoupler | ||
}} | }} | ||
Revision as of 21:36, 21 April 2014
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
MitoPedia topics: Uncoupler
Preparation of 10 mM DNP stock solution (dissolved in H2O):
Caution: Toxic!
- 1. Weigh 3.7 mg of DNP (FW = 184.11).
- 2. Dissolve in 1.2 ml H2O.
- 3. Neutralize with 1 N KOH.
- 4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
- 6. Store at -20 °C.
