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Revision as of 08:19, 6 July 2022 by Gnaiger Erich (talk | contribs) (Created page with "{{MitoPedia |abbr=DHQ |description=ET-pathway level 2 is stimulated by '''duroquinol''' (DHQ) feeding electrons into Complex III (CIII) with further electron transfer to CIV a...")
- high-resolution terminology - matching measurements at high-resolution
Duroquinol
Description
ET-pathway level 2 is stimulated by duroquinol (DHQ) feeding electrons into Complex III (CIII) with further electron transfer to CIV and oxygen.
Abbreviation: DHQ
Reference: Gnaiger 2020 MitoPathways
Application in HRR
Communicated by Axelrod Christopher L 2022-07-06
- We buy tetramethylhydroquinone (duroquinol) manufactured by TCI. This can be purchased directly from TCI (https://www.tcichemicals.com/US/en/search/?text=T0822) or from Fisher Scientific (https://www.fishersci.com/shop/products/tetramethylhydroquinone-tci-america-2/T08225G#?keyword=T0822).
- DHQ should be stored at room temperature protected from light. The stock solution needs to be prepared fresh for each experiment. We prepare a 0.1 M stock solution and inject to a final concentration of 1 mM. Depending on the tissue, this can be decreased to 0.5 mM.
- Normally, we titrate FCCP in the presence of DHQ to determine CIII-linked ET capacity. Depending on the tissue kinetics, this may need to occur immediately once the peak has been obtained (similar to As/Tm).
- We have extensively validated this product against the DHQ synthesized by our group - it works very well.
MitoPedia O2k and high-resolution respirometry: O2k-Respirometry
MitoPedia topics:
Substrate and metabolite
