Respiratory electron transfer system capacity of mitochondria in the experimentally controlled state of non-coupled respiration (fully uncoupled; state E), obtained in intact cells or mitochondrial preparations with defined substrates, by titration of an established uncoupler up to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of ETS capacity. In this state E, the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and for measurement of the mt-membrane potential.
References
- Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 1837–1845. - link
- Gnaiger E (2010) MitoPathways: Respiratory states and flux control ratios. Mitochondr. Physiol. Network 12.15: 1-5. - link
- high-resolution terminology - matching measurements at high-resolution
ET capacity
