Difference between revisions of "GM-pathway control state"
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|description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]]. | |description=[[File:GM.jpg|left|200px|GM]] '''GM''': [[Glutamate]] & [[Malate]]. | ||
'''MitoPathway control state:''' N | '''MitoPathway control state:''' [[N|NADH Electron transfer-pathway state]] | ||
When glutamate&malate are added to isolated mitochondria or permeabilized cells, glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase. | When glutamate&malate are added to isolated mitochondria or permeabilized cells, glutamate and transaminase are responsible for the metabolism of [[oxaloacetate]], comparable to the metabolism with acetyl-CoA and citrate synthase. | ||
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|mitopedia concept=SUIT state | |mitopedia concept=SUIT state | ||
}} | }} | ||
::: ''More details'' | |||
::::» [[NADH Electron transfer-pathway state]] | |||
== GM<sub>''L''</sub> == | |||
GM pathway in the LEAK state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-011]] | |||
:::*[[SUIT-014]] | |||
== GM<sub>''P''</sub> == | |||
GM pathway in the OXPHOS state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-007]] | |||
::::* DL-Protocol for permeabilized cells (pce): | |||
:::*[[SUIT-011]] | |||
:::*[[SUIT-014]] | |||
== GM<sub>''E''</sub> == | |||
GM pathway in the ET state can be evaluated in the following SUIT protocols: | |||
:::*[[SUIT-007]] | |||
::::* DL-Protocol for permeabilized cells (pce): | |||
== Discussion == | == Discussion == | ||
:::: In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GM<sub>''P''</sub>) is identical or 10% higher than with [[PM]] (PM<sub>''P''</sub>). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PM<sub>''P''</sub> being 16% to 25% higher than for GM<sub>''P''</sub>. In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>. | :::: In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GM<sub>''P''</sub>) is identical or 10% higher than with [[PM]] (PM<sub>''P''</sub>). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PM<sub>''P''</sub> being 16% to 25% higher than for GM<sub>''P''</sub>. In fibroblasts, GM<sub>''P''</sub> supports a higher respiratory flux than PM<sub>''P''</sub>. | ||
Revision as of 16:45, 22 January 2019
- high-resolution terminology - matching measurements at high-resolution
GM-pathway control state
Description
MitoPathway control state: NADH Electron transfer-pathway state
When glutamate&malate are added to isolated mitochondria or permeabilized cells, glutamate and transaminase are responsible for the metabolism of oxaloacetate, comparable to the metabolism with acetyl-CoA and citrate synthase.
Abbreviation: GM
Reference: Gnaiger 2014 MitoPathways - Chapter 3.4
MitoPedia concepts:
SUIT state
- More details
GML
GM pathway in the LEAK state can be evaluated in the following SUIT protocols:
GMP
GM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
GME
GM pathway in the ET state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for permeabilized cells (pce):
-
Discussion
- In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GMP) is identical or 10% higher than with PM (PMP). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PMP being 16% to 25% higher than for GMP. In fibroblasts, GMP supports a higher respiratory flux than PMP.
