Difference between revisions of "Glucose"
From Bioblast
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|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
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== Application in [[ | == Application in [[HRR]] == | ||
'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at | '''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; FW = 180.16 | ||
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::1) weigh 360.32 mg of Glucose | ::1) weigh 360.32 mg of Glucose | ||
::2) dissolve 1 | ::2) dissolve 1 mL H<sub>2</sub>O in 1.5 mL Eppendorf | ||
'''Oxygraph-2k Titration:''' | '''Oxygraph-2k Titration:''' | ||
::* Titration volume: 20 | ::* Titration volume: 20 µL using a 50 µL syringe | ||
::* Final conc. in 2 | ::* Final conc. in 2 mL O2k-chamber: 20 mM | ||
Line 29: | Line 29: | ||
::1) weigh 79.2 mg of Glucose | ::1) weigh 79.2 mg of Glucose | ||
::2) dissolve 0.2 | ::2) dissolve 0.2 mL H<sub>2</sub>O in 0.5 mL Eppendorf | ||
'''Oxygraph-2k Titration:''' | '''Oxygraph-2k Titration:''' | ||
::* Titration volume: 5 | ::* Titration volume: 5 µL using a 25 µL syringe | ||
::* Final conc. in 2 | ::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | ||
::* Titration volume: 10 | ::* Titration volume: 10 µL using a 25 µL syringe | ||
::* Final conc. in 2 | ::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) |
Revision as of 13:37, 17 March 2019
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
MitoPedia topics: Substrate and metabolite
Application in HRR
Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; FW = 180.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 360.32 mg of Glucose
- 2) dissolve 1 mL H2O in 1.5 mL Eppendorf
Oxygraph-2k Titration:
- Titration volume: 20 µL using a 50 µL syringe
- Final conc. in 2 mL O2k-chamber: 20 mM
Preparation of 2.2 M stock solution (dissolved in H2O):
- 1) weigh 79.2 mg of Glucose
- 2) dissolve 0.2 mL H2O in 0.5 mL Eppendorf
Oxygraph-2k Titration:
- Titration volume: 5 µL using a 25 µL syringe
- Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L)
- Titration volume: 10 µL using a 25 µL syringe
- Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L)