Difference between revisions of "Glucose"
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== Application in [[ | == Application in [[HRFR]] == | ||
'''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at R.T.; FW = 180.16 | '''Glc: Glucose''' (D-(+)-Glucose, C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at R.T.; FW = 180.16 |
Revision as of 14:16, 29 August 2017
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
MitoPedia topics: Substrate and metabolite
Application in HRFR
Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at R.T.; FW = 180.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 360.32 mg of Glucose
- 2) dissolve 1 ml H2O in 1,5 ml Eppendorf
Oxygraph-2k Titration:
- Titration volume: 20 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 20 mM
Preparation of 2.2 M stock solution (dissolved in H2O):
- 1) weigh 79.2 mg of Glucose
- 2) dissolve 0.2 ml H2O in 0,5 ml Eppendorf
Oxygraph-2k Titration:
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 5.5 mM (1 g/L)
- Titration volume: 10 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 11 mM (2 g/L)