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Difference between revisions of "Gnaiger 2008 POS"

From Bioblast
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|journal=John Wiley
|journal=John Wiley
|mipnetlab=AT Innsbruck GnaigerE,
|mipnetlab=AT Innsbruck GnaigerE,
|abstract=Several features distinguish '''[[high-resolution respirometry]]''' from traditional oxygraphs, combined in the new [[Oxygraph-2k]].
|info=[[http://www.oroboros.at/fileadmin/user_upload/Reprints/2008_Gnaiger_DrugInducedMitoDysfunction.pdf ''pdf'']]
|info=[[http://www.oroboros.at/fileadmin/user_upload/Reprints/2008_Gnaiger_DrugInducedMitoDysfunction.pdf ''pdf'']]
}}
}}
{{Labeling
{{Labeling
|instruments=Oxygraph-2k
|discipline=Mitochondrial Physiology
|organism=Mouse
|tissues=Blood Cell; Suspension Culture
|preparations=Intact Cell; Cultured; Primary
|instruments=Oxygraph-2k, TIP2k, DatLab Software; Separate Application, Method
}}
}}
===Highlights===
===Highlights===
Several features distinguish '''[[high-resolution respirometry]]''' from traditional oxygraphs, combined in the new [[Oxygraph-2k]] (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-­1∙cm-­3 (0.001 µM∙s-­1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers.
Several features distinguish '''[[high-resolution respirometry]]''' from traditional oxygraphs, combined in the new [[Oxygraph-2k]] (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-­1∙cm-­3 (0.001 µM∙s-­1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers.

Revision as of 19:04, 2 October 2010

Publications in the MiPMap
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.

» [pdf]

Gnaiger E (2008) John Wiley

Abstract: Several features distinguish high-resolution respirometry from traditional oxygraphs, combined in the new Oxygraph-2k.


O2k-Network Lab: AT Innsbruck GnaigerE


Labels:


Organism: Mouse  Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k, TIP2k, DatLab Software; Separate Application"DatLab Software; Separate Application" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Method"Method" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 


Highlights

Several features distinguish high-resolution respirometry from traditional oxygraphs, combined in the new Oxygraph-2k (O2k, OROBOROS INSTRUMENTS, Innsbruck, Austria; Figure 3). The specifications are unique: the limit of detection of respiratory flux is 1 pmol∙s-­1∙cm-­3 (0.001 µM∙s-­1) and the limit of detection of oxygen concentration extends to 0.005 µM O2. For the non-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of respiratory activity in each metabolic state and to permit complex titration regimes in intact cells or in permeabilized cells and tissues. To increase throughput in research with cell cultures and in the pharmacological arena, user-friendly features make it possible to apply several instruments in parallel, each O2k with two independent chambers.