Difference between revisions of "Guzun 2011 Biochim Biophys Acta"
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{{Publication | {{Publication | ||
|title=Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremäe M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Mitochondria–cytoskeleton interaction: Distribution of β-tubulins in cardiomyocytes and HL-1 cells. Biochim Biophys Acta 1807: 458- | |title=Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremäe M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Mitochondria–cytoskeleton interaction: Distribution of β-tubulins in cardiomyocytes and HL-1 cells. Biochim Biophys Acta 1807:458-69. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21296049 PMID: 21296049] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/21296049 PMID: 21296049] | ||
|authors=Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, | |authors=Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremaee M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V | ||
|year=2011 | |year=2011 | ||
|journal=Biochim Biophys Acta | |journal=Biochim Biophys Acta | ||
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|tissues=Heart | |tissues=Heart | ||
|model cell lines=Other cell lines | |model cell lines=Other cell lines | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|substratestates=CI | |substratestates=CI | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Revision as of 12:23, 24 February 2015
Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremäe M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Mitochondria–cytoskeleton interaction: Distribution of β-tubulins in cardiomyocytes and HL-1 cells. Biochim Biophys Acta 1807:458-69. |
Guzun R, Karu-Varikmaa M, Gonzalez-Granillo M, Kuznetsov AV, Michel L, Cottet-Rousselle C, Saaremaee M, Kaambre T, Metsis M, Grimm M, Auffray C, Saks V (2011) Biochim Biophys Acta
Abstract: Mitochondria-cytoskeleton interactions were analyzed in adult rat cardiomyocytes and in cancerous non-beating HL-1 cells of cardiac phenotype. We show that in adult cardiomyocytes βII-tubulin is associated with mitochondrial outer membrane (MOM). βI-tubulin demonstrates diffused intracellular distribution, βIII-tubulin is colocalized with Z-lines and βIV-tubulin forms microtubular network. HL-1 cells are characterized by the absence of βII-tubulin, by the presence of bundles of filamentous βIV-tubulin and diffusely distributed βI- and βIII-tubulins. Mitochondrial isoform of creatine kinase (MtCK), highly expressed in cardiomyocytes, is absent in HL-1 cells. Our results show that high apparent K(m) for exogenous ADP in regulation of respiration and high expression of MtCK both correlate with the expression of βII-tubulin. The absence of βII-tubulin isotype in isolated mitochondria and in HL-1 cells results in increased apparent affinity of oxidative phosphorylation for exogenous ADP. This observation is consistent with the assumption that the binding of βII-tubulin to mitochondria limits ADP/ATP diffusion through voltage-dependent anion channel of MOM and thus shifts energy transfer via the phosphocreatine pathway. On the other hand, absence of both βII-tubulin and MtCK in HL-1 cells can be associated with their more glycolysis-dependent energy metabolism which is typical for cancer cells (Warburg effect).
• O2k-Network Lab: EE Tallinn Kaambre T, EE Tallinn Saks VA, FR Grenoble Saks VA, FR Grenoble Schlattner U
Labels: MiParea: Respiration, mt-Structure;fission;fusion
Organism: Rat
Tissue;cell: Heart
Preparation: Isolated mitochondria
Coupling state: OXPHOS
HRR: Oxygraph-2k