Hedges IBEC2012
Hedges CP, Hickey AJ, McMahon CD, Smith HK (2012) A fibre-type-specific role for myostatin in regulating mitochondrial function. IBEC2012. |
Link:
Hedges C, Hickey AJ, McMahon CD, Smith HK (2012)
Event: 15th International Biochemistry of Exercise Conference
Aim: This study aimed to compare mitochondrial oxygen consumption in C57BL/6J wild-type and myostatin-deficient mouse soleus and white gastrocnemius muscles.
Methods: Muscles were obtained from 4 month-old male mice. Mass-specific oxygen consumption (pmol.O2/mg/sec) was measured in permeabilised muscle, using high-resolution respirometry (Oroboros Oxygraph-2k). Maximal activities of citrate synthase and lactate dehydrogenase were determined by spectrophotometry.
Results: Myostatin-deficient soleus (n=6) consumed 20% more oxygen per mg than wild-type soleus (n=6) during oxidative phosphorylation. This was accompanied by greater citrate synthase and lactate dehydrogenase enzyme activity in myostatin-deficient soleus (29% and 80% respectively). Myostatin-deficient gastrocnemius (n=7) showed 26% lower oxygen consumption during uncoupled respiration, and 34% lower oxygen consumption when oxidising glycerol-3-phosphate compared to wild-type gastrocnemius (n=8). Citrate synthase activity was not significantly different and lactate dehydrogenase activity 26% greater in myostatin-deficient gastrocnemius compared to wild-type gastrocnemius.
Conclusion: These data suggest that myostatin-deficiency exerts fibre-type specific effects on skeletal muscle mitochondrial function.
β’ O2k-Network Lab: NZ Auckland Hickey AJ, AU Melbourne Stepto NK
Labels: MiParea: Respiration, Genetic knockout;overexpression, Comparative MiP;environmental MiP
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Enzyme: TCA Cycle and Matrix Dehydrogenases"TCA Cycle and Matrix Dehydrogenases" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k, Spectrophotometry"Spectrophotometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.