Difference between revisions of "Horseradish peroxidase"
From Bioblast
| Line 10: | Line 10: | ||
{{MitoPedia topics|type=Enzyme | {{MitoPedia topics|type=Enzyme | ||
}} | }} | ||
== Application in [[ | == Application in [[HRFR]] == | ||
'''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C | ::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C. | ||
:::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]]. | |||
[[ | |||
:::: [[Amplex_ultrared|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex ultrared]] | |||
== Reaction == | == Reaction == | ||
Donor + H<sub>2</sub>O<sub>2</sub> → Oxidized Donor + 2 H<sub>2</sub>O | :::: Donor + H<sub>2</sub>O<sub>2</sub> → Oxidized Donor + 2 H<sub>2</sub>O | ||
== Known inhibitors == | |||
:::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions. | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:00, 22 August 2017
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRFR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
