Difference between revisions of "Horseradish peroxidase"
From Bioblast
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|description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | |description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
}} | }} | ||
{{ | {{Keywords H2O2}} | ||
== Application in [[HRFR]] == | == Application in [[HRFR]] == | ||
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'''Preparation of 500 U/ml stock solution''' (dissolved in MiR05): | '''Preparation of 500 U/ml stock solution''' (dissolved in MiR05): | ||
:: | ::::# Weigh 3,33 mg | ||
:: | ::::# Dissolve in 1 mL MiR05. | ||
:: | ::::# Aliquot 200 µL into 500 µL Eppendorf tubes. | ||
:::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]]. | :::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]]. | ||
Revision as of 02:59, 26 November 2018
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRFR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
Preparation of 500 U/ml stock solution (dissolved in MiR05):
- Weigh 3,33 mg
- Dissolve in 1 mL MiR05.
- Aliquot 200 µL into 500 µL Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
