Difference between revisions of "Horseradish peroxidase"
From Bioblast
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|abbr=HRP | |abbr=HRP | ||
|description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | |description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | ||
|type=Enzyme | |type=Enzyme | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
|type=Enzyme | |||
}} | |||
{{MitoPedia topics|type=Enzyme | |||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 15:08, 24 May 2012
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRR
HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C
Reaction
Donor + H2O2 → Oxidized Donor + 2 H2O
Comment
The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.
