Difference between revisions of "Horseradish peroxidase"
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Donor + H<sub>2</sub>O<sub>2</sub> β Oxidized Donor + 2 H<sub>2</sub>O | Donor + H<sub>2</sub>O<sub>2</sub> β Oxidized Donor + 2 H<sub>2</sub>O | ||
== Known Inhibitors == | |||
according to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigam-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd | |||
Ed., Part A: 367-368.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions | |||
== Comment == | == Comment == | ||
The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by [[Tretter L|Laszlo Tretter]]. | The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by [[Tretter L|Laszlo Tretter]]. | ||
== References == | |||
<references/> |
Revision as of 09:13, 6 June 2012
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRR
HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 Β°C
Reaction
Donor + H2O2 β Oxidized Donor + 2 H2O
Known Inhibitors
according to Sigam-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions
Comment
The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.
References
- β Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd Ed., Part A: 367-368.