Difference between revisions of "Horseradish peroxidase"

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Horseradish peroxidase

Description

Horseradish peroxidase readily combines with hydrogen peroxide (H₂O₂) and the resultant [HRP-H₂O₂] complex can oxidize a wide variety of hydrogen donors.

Abbreviation: HRP

MitoPedia methods: Fluorometry

Application in HRR

HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C

Reaction

Donor + H₂O₂ → Oxidized Donor + 2 H₂O

Known Inhibitors

according to Sigma-Adrich, citing ^[1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, and Pb²⁺ ions.

Comment

The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.

References

↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd Ed., Part A: 367-368.

[1] Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd Ed., Part A: 367-368.

[1]

@@ Line 21: / Line 21: @@
 == Known Inhibitors ==
-according to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigam-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd
+according to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd
 Ed., Part A: 367-368.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions.