Difference between revisions of "Horseradish peroxidase"
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'''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C | '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C | ||
== Application in [[fluorometry]] == | |||
[[Amplex_red|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex Red]] | |||
Revision as of 12:41, 6 March 2014
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRR
HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C
Application in fluorometry
Measurement of H2O2 with Amplex Red
Reaction
Donor + H2O2 → Oxidized Donor + 2 H2O
Known Inhibitors
according to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
Comment
The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd Ed., Part A: 367-368.
