Difference between revisions of "Horseradish peroxidase"
From Bioblast
Line 12: | Line 12: | ||
::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C. | ::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C. | ||
'''Preparation of 500 U/ml stock solution''' (dissolved in MiR05): | |||
::1) Weigh 3,33 mg | |||
::2) Dissolve in 1 ml MiR05. | |||
::3) Aliquot 200µl into 500µl Eppendorf tubes. | |||
:::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]]. | :::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]]. |
Revision as of 11:55, 3 May 2018
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods: Fluorometry
Application in HRFR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
Preparation of 500 U/ml stock solution (dissolved in MiR05):
- 1) Weigh 3,33 mg
- 2) Dissolve in 1 ml MiR05.
- 3) Aliquot 200µl into 500µl Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.