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Horseradish peroxidase

From Bioblast


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Horseradish peroxidase

Description

Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.

Abbreviation: HRP


MitoPedia topics: Enzyme 


Application in HRR

HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 Β°C


Reaction

Donor + H2O2 β†’ Oxidized Donor + 2 H2O

Comment

The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by [[ Tretter L|Laszlo Tretter]].